Review



anti-apoe antibody polyclonal goat  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Millipore anti-apoe antibody polyclonal goat
    Anti Apoe Antibody Polyclonal Goat, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+goat+anti-apoe+antibody/goat+anti+apoe/pm40387921-257-41-52
    Average 90 stars, based on 1 article reviews
    anti-apoe antibody polyclonal goat - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Incubation:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Expressing:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Immunostaining:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Binding Assay:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Amplification:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Western Blot:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Double Immunostaining:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Blocking Assay:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Affinity Purification:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Staining:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    SDS Page:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Membrane:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Clear Native PAGE:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Fluorescence:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Residue:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Emulsion:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Gentle:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Centrifugation:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Cell Culture:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Clinical Proteomics:

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [8]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 April 2015 Volume 89 Number 8 jvi.asm.org 4563Journal of Virology (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1: 2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia
    Article Snippet: Human apoE3 levels were quantified by an ELISA using polyclonal goat anti-apoE antibody (Chemicon, 1:1000) and apoE3 (Calbiochem) as standard [ 18 ].

    Article Title: Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function in Huntington's disease
    Article Snippet: They were then probed with a polyclonal goat anti-apoE antibody (1 : 800; AB947, Millipore), washed, and probed with HRP-conjugated horse anti-goat IgG (1 : 3000; Bio-Rad).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse antiGFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1: 1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).

    Article Title: VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma Lipids
    Article Snippet: The primary antibodies were polyclonal goat anti-ApoE antibody (Millipore, Billerica, MA) and affinity-purified rabbit anti-VPO1 antibody as described in the article .

    Article Title: Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
    Article Snippet: The membranes were then probed at 4°C overnight with primary antibodies for Core (monoclonal mouse anti-Core antibody) (clone C7-50; Thermo Scientific) (1:2,000 dilution), NS2 (polyclonal rabbit anti-NS2 antibody [ 8 ]) (1:15,000 dilution), E2 (polyclonal goat anti-E2 antibody; Virostat, Inc., Portland, ME) (1:2,000 dilution), NS3 (monoclonal mouse anti-NS3 antibody) (clone 9-G2; ViroGen, Watertown, MA) (1:2,000 dilution), Calnexin (polyclonal rabbit anticalnexin antibody; Calbiochem) (1:5,000 dilution), Flotilin-1 (monoclonal mouse anti-flotilin-1; BD Transduction Laboratories) (1:2,000 dilution), SPFH2 (also known as Erlin-2 [endoplasmic reticulum lipid raft protein 2]; polyclonal goat anti-SPFH2 antibody, Santa Cruz Biotechnology Inc.) (1:2,000 dilution), and apolipoprotein E (ApoE) (polyclonal goat anti-ApoE antibody; Chemicon International) (1:3,000 dilution).

    Article Title: Isoform- and cell type-specific structure of apolipoprotein E lipoparticles as revealed by a novel Forster resonance energy transfer assay
    Article Snippet: The CM was then concentrated 15-fold using 10KDa 15mL Amicon concentration columns (#UFC901024, Millipore) and was attached to protein G Dybabeads (#10007D, ThermoFisher) overnight at 4 o C with rotation using 4ul of a polyclonal goat anti-ApoE antibody (#AB947, Millipore).

    Article Title: Apolipoprotein E4 Produced in GABAergic Interneurons Causes Learning and Memory Deficits in Mice
    Article Snippet: For immunofluorescence analysis, the following primary antibodies were incubated overnight at 4°C: polyclonal goat anti-apoE antibody (1:5000; Calbiochem), polyclonal rabbit anti-Cre antibody (1:5000; Novagen), monoclonal mouse anti- NeuN antibody (1:2000; Millipore), polyclonal rabbit anti- GFAP antibody (1:2000; DAKO), monoclonal mouse anti-GFAP (1:2000; Millipore), polyclonal rabbit anti-GABA antibody (1:1000; Sigma), polyclonal rabbit anti-somatostatin antibody (1:500; Bachem).



    Similar Products

    90
    Millipore anti-apoe antibody polyclonal goat
    Anti Apoe Antibody Polyclonal Goat, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+goat+anti-apoe+antibody/goat+anti+apoe/pm40387921-257-41-52
    Average 90 stars, based on 1 article reviews
    anti-apoe antibody polyclonal goat - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Meridian Life Science goat polyclonal anti-apoe antibody
    Goat Polyclonal Anti Apoe Antibody, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+goat+anti-apoe+antibody/biotin+conjugated+goat+anti+apoe+antibody/pm39380021-68-15-22
    Average 90 stars, based on 1 article reviews
    goat polyclonal anti-apoe antibody - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Bio-Techne corporation apolipoprotein a-i/apoa1 antibody
    Apolipoprotein A I/Apoa1 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+goat+anti-apoe+antibody/Apolipoprotein+A-I%2FApoA1+Antibody/bio-techne+corporation___nb400-147
    Average 90 stars, based on 1 article reviews
    apolipoprotein a-i/apoa1 antibody - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Millipore goat polyclonal anti-apoe primary antibody
    Goat Polyclonal Anti Apoe Primary Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+goat+anti-apoe+antibody/goat+anti+apoe/pm38871936-83-12-17
    Average 90 stars, based on 1 article reviews
    goat polyclonal anti-apoe primary antibody - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    86
    Danaher Inc polyclonal goat anti apo a capture antibody
    Polyclonal Goat Anti Apo A Capture Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+goat+anti-apoe+antibody/pmc11111404-136-12-17
    Average 86 stars, based on 1 article reviews
    polyclonal goat anti apo a capture antibody - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    90
    Meridian Life Science goat anti-apoe polyclonal antibody
    <t>ApoE</t> size distribution of fresh and frozen APOE3/3 immortalized astrocyte conditioned media (iAS CM) Three independent APOE3/3 immortalized astrocyte batches were grown to confluency in 182.0 cm 2 growth flasks and conditioned in 20 mL of 2%-FBS containing media for 72 h prior to collection. The media was split into two 10 mL aliquots, one of which was immediately frozen at −80°C, and the other was utilized for SEC. After 48 h, the frozen sample was thawed at 4°C and utilized for SEC. WUE-4 ELISA was performed using fractions 18–56 for both samples, and the size was calculated using commercial protein standard (A). The fractions were then divided into tertiles defined by size (≥615 kDa–20 kDa≥). The area under the curve (AUC) was calculated and paired t-tests (two-tailed) were performed utilizing GraphPad Prism 9.4.1. Data are represented as mean ± SEM. P < 0.05 is considered statistically significant. N.S. = Not significant (B).
    Goat Anti Apoe Polyclonal Antibody, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+goat+anti-apoe+antibody/apob+antibody/pmc10323120-5-0-6
    Average 90 stars, based on 1 article reviews
    goat anti-apoe polyclonal antibody - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Academy Bio-Medical polyclonal goat anti-human apoe antibody
    <t>ApoE</t> size distribution of fresh and frozen APOE3/3 immortalized astrocyte conditioned media (iAS CM) Three independent APOE3/3 immortalized astrocyte batches were grown to confluency in 182.0 cm 2 growth flasks and conditioned in 20 mL of 2%-FBS containing media for 72 h prior to collection. The media was split into two 10 mL aliquots, one of which was immediately frozen at −80°C, and the other was utilized for SEC. After 48 h, the frozen sample was thawed at 4°C and utilized for SEC. WUE-4 ELISA was performed using fractions 18–56 for both samples, and the size was calculated using commercial protein standard (A). The fractions were then divided into tertiles defined by size (≥615 kDa–20 kDa≥). The area under the curve (AUC) was calculated and paired t-tests (two-tailed) were performed utilizing GraphPad Prism 9.4.1. Data are represented as mean ± SEM. P < 0.05 is considered statistically significant. N.S. = Not significant (B).
    Polyclonal Goat Anti Human Apoe Antibody, supplied by Academy Bio-Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+goat+anti-apoe+antibody/goat+anti+apoa+i+polyclonal+antibody/pm37221560-62-14-19
    Average 90 stars, based on 1 article reviews
    polyclonal goat anti-human apoe antibody - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Millipore antibodies goat polyclonal anti-apolipoprotein e (apoe)
    <t>ApoE</t> size distribution of fresh and frozen APOE3/3 immortalized astrocyte conditioned media (iAS CM) Three independent APOE3/3 immortalized astrocyte batches were grown to confluency in 182.0 cm 2 growth flasks and conditioned in 20 mL of 2%-FBS containing media for 72 h prior to collection. The media was split into two 10 mL aliquots, one of which was immediately frozen at −80°C, and the other was utilized for SEC. After 48 h, the frozen sample was thawed at 4°C and utilized for SEC. WUE-4 ELISA was performed using fractions 18–56 for both samples, and the size was calculated using commercial protein standard (A). The fractions were then divided into tertiles defined by size (≥615 kDa–20 kDa≥). The area under the curve (AUC) was calculated and paired t-tests (two-tailed) were performed utilizing GraphPad Prism 9.4.1. Data are represented as mean ± SEM. P < 0.05 is considered statistically significant. N.S. = Not significant (B).
    Antibodies Goat Polyclonal Anti Apolipoprotein E (Apoe), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+goat+anti-apoe+antibody/goat+anti+apoe/pmc10615847__mmc2-761-2-11
    Average 90 stars, based on 1 article reviews
    antibodies goat polyclonal anti-apolipoprotein e (apoe) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    ApoE size distribution of fresh and frozen APOE3/3 immortalized astrocyte conditioned media (iAS CM) Three independent APOE3/3 immortalized astrocyte batches were grown to confluency in 182.0 cm 2 growth flasks and conditioned in 20 mL of 2%-FBS containing media for 72 h prior to collection. The media was split into two 10 mL aliquots, one of which was immediately frozen at −80°C, and the other was utilized for SEC. After 48 h, the frozen sample was thawed at 4°C and utilized for SEC. WUE-4 ELISA was performed using fractions 18–56 for both samples, and the size was calculated using commercial protein standard (A). The fractions were then divided into tertiles defined by size (≥615 kDa–20 kDa≥). The area under the curve (AUC) was calculated and paired t-tests (two-tailed) were performed utilizing GraphPad Prism 9.4.1. Data are represented as mean ± SEM. P < 0.05 is considered statistically significant. N.S. = Not significant (B).

    Journal: STAR Protocols

    Article Title: Standardized immunoprecipitation protocol for efficient isolation of native apolipoprotein E particles utilizing HJ15.4 monoclonal antibody

    doi: 10.1016/j.xpro.2023.102271

    Figure Lengend Snippet: ApoE size distribution of fresh and frozen APOE3/3 immortalized astrocyte conditioned media (iAS CM) Three independent APOE3/3 immortalized astrocyte batches were grown to confluency in 182.0 cm 2 growth flasks and conditioned in 20 mL of 2%-FBS containing media for 72 h prior to collection. The media was split into two 10 mL aliquots, one of which was immediately frozen at −80°C, and the other was utilized for SEC. After 48 h, the frozen sample was thawed at 4°C and utilized for SEC. WUE-4 ELISA was performed using fractions 18–56 for both samples, and the size was calculated using commercial protein standard (A). The fractions were then divided into tertiles defined by size (≥615 kDa–20 kDa≥). The area under the curve (AUC) was calculated and paired t-tests (two-tailed) were performed utilizing GraphPad Prism 9.4.1. Data are represented as mean ± SEM. P < 0.05 is considered statistically significant. N.S. = Not significant (B).

    Article Snippet: Goat anti-apoE polyclonal antibody (1:5000) , Meridian Life Science , Cat#K74180B; RRID: AB_150544.

    Techniques: Enzyme-linked Immunosorbent Assay, Two Tailed Test

    HJ15.4 relative apoE pull-down and elution efficiency APOE3/3 immortalized astrocytes were grown to confluency in two 182.0 cm 2 growth flask and conditioned in 20 mL of 2%-FBS containing media for 72 h prior to collection. The conditioned media was concentrated 20× and 1 mL of media (Input) was added to 50 μL of HJ15.4 monoclonal mouse anti-apoE antibody (HJ15.4) or the mouse IgG isotype control (mIgG) conjugated beads for IP, as described above. The beads were split into 25 μL aliquots following washing (step 25) and were eluted with 4 bead volumes of 3 M NaSCN (NaSCN EL) or 0.1 M glycine pH 2.5 (Gly EL). The eluent was collected, and the beads were resuspended in 1× SDS-containing sample buffer to elute any remaining apoE lipoprotein particles (NaSCN B and Gly B). A western blot was performed utilizing 1% of each respective sample, and K74180B (1:2000) was used for primary antibody detection (A). The membrane was imaged, and signal strength was quantified using Odyssey® CLx Infrared Imaging System by LI-COR Biosciences (B). Pull-down efficiency is estimated by calculating the percentage of apoE remaining in the supernatant sample (SN) post-16-h incubation compared to the input sample. Elution efficiency has been defined as the difference in apoE quantity pulled down and quantity of apoE eluted.

    Journal: STAR Protocols

    Article Title: Standardized immunoprecipitation protocol for efficient isolation of native apolipoprotein E particles utilizing HJ15.4 monoclonal antibody

    doi: 10.1016/j.xpro.2023.102271

    Figure Lengend Snippet: HJ15.4 relative apoE pull-down and elution efficiency APOE3/3 immortalized astrocytes were grown to confluency in two 182.0 cm 2 growth flask and conditioned in 20 mL of 2%-FBS containing media for 72 h prior to collection. The conditioned media was concentrated 20× and 1 mL of media (Input) was added to 50 μL of HJ15.4 monoclonal mouse anti-apoE antibody (HJ15.4) or the mouse IgG isotype control (mIgG) conjugated beads for IP, as described above. The beads were split into 25 μL aliquots following washing (step 25) and were eluted with 4 bead volumes of 3 M NaSCN (NaSCN EL) or 0.1 M glycine pH 2.5 (Gly EL). The eluent was collected, and the beads were resuspended in 1× SDS-containing sample buffer to elute any remaining apoE lipoprotein particles (NaSCN B and Gly B). A western blot was performed utilizing 1% of each respective sample, and K74180B (1:2000) was used for primary antibody detection (A). The membrane was imaged, and signal strength was quantified using Odyssey® CLx Infrared Imaging System by LI-COR Biosciences (B). Pull-down efficiency is estimated by calculating the percentage of apoE remaining in the supernatant sample (SN) post-16-h incubation compared to the input sample. Elution efficiency has been defined as the difference in apoE quantity pulled down and quantity of apoE eluted.

    Article Snippet: Goat anti-apoE polyclonal antibody (1:5000) , Meridian Life Science , Cat#K74180B; RRID: AB_150544.

    Techniques: Control, Western Blot, Membrane, Imaging, Incubation

    Comparison of apoE lipoprotein particle size distribution in APOE3/3 immortalized astrocyte conditioned media (iAS CM Pre-IP) and purified apoE lipoprotein particles in elution reagent following HJ15.4 IP protocol (iAS CM Post-IP) Three independent APOE3/3 immortalized astrocyte batches were grown to confluency in 182.0 cm 2 flasks, and the apoE IP protocol was performed as described above. Following elution, SEC of both the pre-IP and the post-IP samples was performed, and WUE-4 ELISA was used to quantify apoE concentrations of the selected fractions (A). The fraction size was calculated using a commercially available protein standard and the fractions were then divided into tertiles defined by size (≥615 kDa–20 kDa≥). The area under the curve (AUC) of each tertile was calculated and paired t-tests (two-tailed) were performed utilizing GraphPad Prism 9.4.1 (B). Data are represented as mean ± SEM. P < 0.05 is considered statistically significant. N.S. = Not significant. Lastly, native PAGE detecting apoE (K74180B; 1:2000) was performed utilizing samples from a single pre-IP and post-IP batch for qualitative validation (C). APOE3/3 immortalized astrocyte conditioned media, iAS CM Pre-IP; supernatant (step 24), SN; Eluted apoE lipoprotein particles, NaSCN EL.

    Journal: STAR Protocols

    Article Title: Standardized immunoprecipitation protocol for efficient isolation of native apolipoprotein E particles utilizing HJ15.4 monoclonal antibody

    doi: 10.1016/j.xpro.2023.102271

    Figure Lengend Snippet: Comparison of apoE lipoprotein particle size distribution in APOE3/3 immortalized astrocyte conditioned media (iAS CM Pre-IP) and purified apoE lipoprotein particles in elution reagent following HJ15.4 IP protocol (iAS CM Post-IP) Three independent APOE3/3 immortalized astrocyte batches were grown to confluency in 182.0 cm 2 flasks, and the apoE IP protocol was performed as described above. Following elution, SEC of both the pre-IP and the post-IP samples was performed, and WUE-4 ELISA was used to quantify apoE concentrations of the selected fractions (A). The fraction size was calculated using a commercially available protein standard and the fractions were then divided into tertiles defined by size (≥615 kDa–20 kDa≥). The area under the curve (AUC) of each tertile was calculated and paired t-tests (two-tailed) were performed utilizing GraphPad Prism 9.4.1 (B). Data are represented as mean ± SEM. P < 0.05 is considered statistically significant. N.S. = Not significant. Lastly, native PAGE detecting apoE (K74180B; 1:2000) was performed utilizing samples from a single pre-IP and post-IP batch for qualitative validation (C). APOE3/3 immortalized astrocyte conditioned media, iAS CM Pre-IP; supernatant (step 24), SN; Eluted apoE lipoprotein particles, NaSCN EL.

    Article Snippet: Goat anti-apoE polyclonal antibody (1:5000) , Meridian Life Science , Cat#K74180B; RRID: AB_150544.

    Techniques: Comparison, Purification, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Clear Native PAGE, Biomarker Discovery

    Lipids associated with apoE lipoprotein particles isolated from APOE3/3 immortalized astrocyte conditioned medium Heatmap (middle panel) shows scaled levels of 55 lipid species measured by mass spectrometry of six independent apoE isolation assays using the HJ15.4 monoclonal mouse anti-apoE antibody (HJ15.4) or the isotype mouse IgG control (IgG). The amount of each lipid species was compared between the HJ15.4 and IgG groups using linear models (right panel). The lipid species with a fold change ≥ 2 and P value < 0.05 are considered apoE lipoprotein particle-associated lipids. The lipids were normalized to volume of CNBr beads for the pull-down assay (25 μL). The fold change and P values comparing the lipid amount in the HJ15.4 and IgG groups were calculated using linear regression models. The analysis was done using R 4.1.2. CE, Cholesterol Ester; SM, Sphingomyelin; PC, Phosphatidylcholine; PE, Phosphatidylethanolamine; LPC, Lysophosphatidylcholine; LPE, Lysophosphatidylethanolamine. ∗∗∗P < 0.001; ∗∗P < 0.01; ∗P < 0.05.

    Journal: STAR Protocols

    Article Title: Standardized immunoprecipitation protocol for efficient isolation of native apolipoprotein E particles utilizing HJ15.4 monoclonal antibody

    doi: 10.1016/j.xpro.2023.102271

    Figure Lengend Snippet: Lipids associated with apoE lipoprotein particles isolated from APOE3/3 immortalized astrocyte conditioned medium Heatmap (middle panel) shows scaled levels of 55 lipid species measured by mass spectrometry of six independent apoE isolation assays using the HJ15.4 monoclonal mouse anti-apoE antibody (HJ15.4) or the isotype mouse IgG control (IgG). The amount of each lipid species was compared between the HJ15.4 and IgG groups using linear models (right panel). The lipid species with a fold change ≥ 2 and P value < 0.05 are considered apoE lipoprotein particle-associated lipids. The lipids were normalized to volume of CNBr beads for the pull-down assay (25 μL). The fold change and P values comparing the lipid amount in the HJ15.4 and IgG groups were calculated using linear regression models. The analysis was done using R 4.1.2. CE, Cholesterol Ester; SM, Sphingomyelin; PC, Phosphatidylcholine; PE, Phosphatidylethanolamine; LPC, Lysophosphatidylcholine; LPE, Lysophosphatidylethanolamine. ∗∗∗P < 0.001; ∗∗P < 0.01; ∗P < 0.05.

    Article Snippet: Goat anti-apoE polyclonal antibody (1:5000) , Meridian Life Science , Cat#K74180B; RRID: AB_150544.

    Techniques: Isolation, Mass Spectrometry, Control, Pull Down Assay

    Journal: STAR Protocols

    Article Title: Standardized immunoprecipitation protocol for efficient isolation of native apolipoprotein E particles utilizing HJ15.4 monoclonal antibody

    doi: 10.1016/j.xpro.2023.102271

    Figure Lengend Snippet:

    Article Snippet: Goat anti-apoE polyclonal antibody (1:5000) , Meridian Life Science , Cat#K74180B; RRID: AB_150544.

    Techniques: Control, Recombinant, Protease Inhibitor, Purification, Coomassie Assay, Software, Sterility